[14]C [FBbi_00000123]
[[14]C; metabolically incorporated radioisotope]
[[14]C; metabolically incorporated radioisotope]
metabolically incorporated radioisotope
[[3]H; metabolically incorporated radioisotope]
metabolically incorporated radioisotope
[[32]P; metabolically incorporated radioisotope]
metabolically incorporated radioisotope
[[32]P labeled DNA probe]
[[32]P labeled RNA probe]
[metabolically incorporated radioisotope; [33]P]
metabolically incorporated radioisotope
[[33]P labeled DNA probe]
[[33]P labeled RNA probe]
[[35]S; metabolically incorporated radioisotope]
metabolically incorporated radioisotope
[3D-deconvolution; linear method]
linear method
[membrane impermeant probe; 4',6-diamidino-2-phenylindole (DAPI)]
[5-SFX fluorescein RNA fill; fluor labeled RNA fill]
fluor labeled RNA fill
[membrane impermeant probe; 7-amino-actinomycin D]
membrane impermeant probe
[imaged parameter; absorption of illumination]
imaged parameter
[AceFP; Green fluorescent proteins from Anthozoa]
Green fluorescent proteins from Anthozoa
[coagulating-fixative fixed tissue; acetone fixed tissue]
coagulating-fixative fixed tissue
[enzyme label; acid phosphatase]
enzyme label
[membrane impermeant probe; acridine homodimer]
membrane impermeant probe
[acridine orange; AO; membrane-permeant probe]
membrane-permeant probe
[acrolein fixed tissue; coagulating-fixative fixed tissue]
coagulating-fixative fixed tissue
[acrylic painted graphic]
painted graphic
[RFP; Aequorea victoria red fluorescent protein tag]
[probe for protein; affinity for specific proteins]
probe for protein
[Alexa Fluor 350 phalloidin fill]
[fluorescent label; Alexa Fluor 488]
fluorescent label
[Alexa Fluor 488 actin fill]
[Alexa Fluor 488 phalloidin fill]
[Alexa Fluor 546; fluorescent label]
fluorescent label
[fluorescent label; Alexa Fluor 555]
fluorescent label
[fluorescent label; Alexa Fluor 568]
fluorescent label
[Alexa Fluor 568 actin fill]
[fluorescent label; Alexa Fluor 594]
fluorescent label
[Alexa Fluor 610; fluorescent label]
fluorescent label
[fluorescent label; Alexa Fluor 633]
fluorescent label
[fluorescent label; Alexa Fluor 647]
fluorescent label
[enzyme label; alkaline phosphatase]
enzyme label
[non-immunological protein probe; alpha-bungarotoxin]
non-immunological protein probe
[AmCyanFP; Cyan fluorescent proteins from Anthozoa]
Cyan fluorescent proteins from Anthozoa
[AMCA; aminomethylcoumarin; fluorescent label]
fluorescent label
Illumination is distributed asymmetrically around the optic axis, typically achieved by a non-circular stop placed below the lower lens element and aperture of the condenser.
Illumination is distributed asymmetrically around the optic axis, typically achieved by a non-circular stop placed below the lower lens element and aperture of the condenser.
A form of graphical illustration that changes with time to give a sense of motion or represent dynamic changes in the portrayal.
A form of graphical illustration that changes with time to give a sense of motion or represent dynamic changes in the portrayal.
scanning probe microscopy
Aperture-less NSOM.
Aperture-less NSOM.
[antibody; macromolecular probe]
macromolecular probe
spot detector
[Far-red fluorescent proteins from Anthozoa; AQ143]
Far-red fluorescent proteins from Anthozoa
[lectin; Arachis hypogaea (PNA)]
[area detector; detection method]
detection method
Multiple points of illumination generated by a fixed array of sources.
Multiple points of illumination generated by a fixed array of sources.
Fixed array of sources focused to an array of spots that is swept over the specimen in raster fashion
Fixed array of sources focused to an array of spots that is swept over the specimen in raster fashion
[AsRed2FP; Red fluorescent proteins from Anthozoa]
Red fluorescent proteins from Anthozoa
the scanning probe is maintained at a fixed distance above the surface e of the specimen by van der Waals forces
the scanning probe is maintained at a fixed distance above the surface e of the specimen by van der Waals forces
[negative staining; aurothioglucose]
negative staining
[spot detector; avalanche photodiode (APD)]
[Green fluorescent proteins from Anthozoa; Azami GreenFP]
Green fluorescent proteins from Anthozoa
[AzuriteFP; Blue fluorescent protein from Aequorea victoria]
Blue fluorescent protein from Aequorea victoria
imaged formed using electrons undergoing elastic scattering at very high angles, with emission of characteristic X-rays
imaged formed using electrons undergoing elastic scattering at very high angles, with emission of characteristic X-rays
[affinity for specific proteins; batrachatoxin]
affinity for specific proteins
[lacZ, X-gal; beta-galactosidase; genetically encoded enzyme]
genetically encoded enzyme
[GUS, X-gluc; beta-glucuronidase; genetically encoded enzyme]
genetically encoded enzyme
[fluorescent label; biotin]
fluorescent label
[miscellaneous fill; biotin ethylenediamine fill; Neurobiotin]
miscellaneous fill
[biotin labeled DNA probe]
[biotin labeled RNA probe]
[black and white graphic; computer graphic]
computer graphic
[3D-deconvolution; blind deconvolution]
3D-deconvolution
[Blue fluorescent protein from Aequorea victoria; fluorescent protein derived from Aequorea victoria]
fluorescent protein derived from Aequorea victoria
[fluorescent protein derived from Anthozoa; Blue fluorescent proteins from Anthozoa]
fluorescent protein derived from Anthozoa
[BODIPY TR-14-dUTP labeled DNA probe]
[botulinum toxin; non-immunological protein probe]
non-immunological protein probe
[boundaries between regions with different refractive index; source of contrast]
source of contrast
[wide-field detection; absorption of illumination; bright-field microscopy; makes_use_of; widefield illumination; light microscopy]
light microscopy
[bromodeoxyuridine labeled DNA probe]
[bsDronpa; photoswitchable fluorescent protein]
photoswitchable fluorescent protein
[c-MYC peptide tag; small genetically encoded tag]
small genetically encoded tag
[graphic illustration; camera lucida assisted graphic]
graphic illustration
coherent Raman anti-Stokes microscopy
coherent Raman anti-Stokes microscopy
[fluorescent label; Cascade blue]
fluorescent label
[cationic colloidal gold; optically-dense stain]
optically-dense stain
area detector
[Cyan fluorescent protein from Aequorea; CeruleanFP]
Cyan fluorescent protein from Aequorea
[charcoal pencil graphic; line art graphic]
line art graphic
[area detector; charge coupled device (CCD)]
[imaged parameter; chemical composition]
imaged parameter
[chemically fixed tissue; fixation method]
fixation method
[cholera toxin B; lectin]
[CitrineFP; Yellow fluorescent protein from Aequorea]
Yellow fluorescent protein from Aequorea
area detector
[Mitotracker X; probe for mitochondria; CMX rosamine (Mitotracker Red)]
probe for mitochondria
[chemically fixed tissue; coagulating-fixative fixed tissue; solvent permeabilized]
chemically fixed tissue; solvent permeabilized
[miscellaneous fill; cobalt fill]
miscellaneous fill
[coherent illumination; illumination method]
illumination method
[color graphic; computer graphic]
computer graphic
[colored pencil graphic; line art graphic]
line art graphic
[source of contrast; compartmentalization of stain or label]
source of contrast
[area detector; complementary metal oxide semiconductor (CMOS)]
[computational method; contrast-enhancing method]
contrast-enhancing method
Tomography in which virtual sections are taken from multiple angles and the results are processed via tomographic reconstruction software to produce 2 or 3D images.
Tomography in which virtual sections are taken from multiple angles and the results are processed via tomographic reconstruction software to produce 2 or 3D images.
[graphic illustration; computer graphic]
graphic illustration
[lectin; conconavalin A]
fluorescence microscopy
[confocal microscopy; fluorescence emission; narrow-field detection; makes_use_of; narrowfield illumination; fluorescence microscopy]
[3D-deconvolution; constrained iterative deconvolution]
3D-deconvolution
[illumination method; continuous illumination]
illumination method
[contrast-enhancing method; method involved in biological imaging]
method involved in biological imaging
[Green fluorescent proteins from Anthozoa; CopGFP]
Green fluorescent proteins from Anthozoa
[electron-dense stain; copper salt]
electron-dense stain
[cresyl fast violet; optically-dense stain]
optically-dense stain
specimen from which water has been removed by a process that avoids liquid-gas and solid-gas phase transitions.
specimen from which water has been removed by a process that avoids liquid-gas and solid-gas phase transitions.
[chemically fixed tissue; crosslinking-fixative fixed tissue]
chemically fixed tissue
[cryofixed tissue; fixation method]
fixation method
[cryostat-sectioned tissue]
sectioned tissue
[fluorescent label; Cy2]
fluorescent label
[Cy3; fluorescent label]
fluorescent label
[fluorescent label; Cy5]
fluorescent label
[Cyan fluorescent protein from Aequorea; fluorescent protein derived from Aequorea victoria]
fluorescent protein derived from Aequorea victoria
[fluorescent protein derived from Anthozoa; Cyan fluorescent proteins from Anthozoa]
fluorescent protein derived from Anthozoa
[CyPet; Cyan fluorescent protein from Aequorea]
Cyan fluorescent protein from Aequorea
membrane impermeant probe
imaging with rejection of the unscattered illumination (removal of the zero order component of the diffracted wave)
imaging with rejection of the unscattered illumination (removal of the zero order component of the diffracted wave)
illumination restricted to angles that exceed the acceptance angle of the image-forming lens
illumination restricted to angles that exceed the acceptance angle of the image-forming lens
[optical method; defocus phase contrast]
optical method
[photoconvertible fluorescent protein; Dendra2FP]
photoconvertible fluorescent protein
[photoconvertible fluorescent protein; dEOSFP]
photoconvertible fluorescent protein
media and devices employed for recording the alterations in the illumination that result from its interaction with the sample
media and devices employed for recording the alterations in the illumination that result from its interaction with the sample
[detection of acoustic waves; detection method]
detection method
[detection method; detection of current induced in the specimen]
detection method
[detection of electrons; detection method]
detection method
[detection of photons; detection method]
detection method
[detection of UV/visible/IR photons; detection of photons]
detection of photons
[detection of X-ray photons; detection of photons]
detection of photons
Membrane permeabilization by the action of ionic or non-ionic detergents
Membrane permeabilization by the action of ionic or non-ionic detergents
[DiA; probe for lipid]
probe for lipid
[diagram; portrayed image]
portrayed image
[probe for lipid; DiD]
probe for lipid
[source of contrast; differences in adsorption or binding of stain]
source of contrast
[differences in amount of elastic light scattering; source of contrast]
source of contrast
[source of contrast; differences in chemical composition]
source of contrast
[source of contrast; differences in deposition of metal shadow]
source of contrast
[differences in fluorescence lifetime; source of contrast]
source of contrast
[source of contrast; differences in intrinsic optical density]
source of contrast
[source of contrast; differences in orientation of fluorophore]
source of contrast
[polarized light illumination; polarization-sensitive detection; differential interference contrast microscopy; optical path length gradient; makes_use_of; Nomarski; DIC; Wollaston prism; light microscopy]
light microscopy
[digoxigenin labeled DNA probe]
[digoxigenin labeled RNA probe]
[DiI; probe for lipid]
probe for lipid
[DiIC16; probe for lipid]
probe for lipid
[DiO; probe for lipid]
probe for lipid
[DiOC6 (3,3’-dihexyloxacarbocyanine iodide); probe for lipid]
probe for lipid
[probe for lipid; DiR]
probe for lipid
[sample preparation method; dispersed cells in vitro]
sample preparation method
[source of contrast; distribution of a specific nucleic acid sequence]
source of contrast
[source of contrast; distribution of a specific protein]
source of contrast
[source of contrast; distribution of epitope]
source of contrast
[DNA probe; sequence-specific nucleic acid probe]
sequence-specific nucleic acid probe
[DOPI (defocussed orientation and position imaging); point-localization method]
point-localization method
[Dronpa; photoswitchable fluorescent protein]
photoswitchable fluorescent protein
[Dronpa-3; photoswitchable fluorescent protein]
photoswitchable fluorescent protein
[DsRed; Orange fluorescent proteins from Anthozoa]
Orange fluorescent proteins from Anthozoa
[DsRed-ES FP; fluorescent protein timer]
fluorescent protein timer
[DsRed-Express2FP; Orange fluorescent proteins from Anthozoa]
Orange fluorescent proteins from Anthozoa
[DsRed-ExpressFP; Orange fluorescent proteins from Anthozoa]
Orange fluorescent proteins from Anthozoa
[DsRed-MaxFP; Orange fluorescent proteins from Anthozoa]
Orange fluorescent proteins from Anthozoa
[DsRed-monomerFP; Orange fluorescent proteins from Anthozoa]
Orange fluorescent proteins from Anthozoa
[DsRed2FP; Orange fluorescent proteins from Anthozoa]
Orange fluorescent proteins from Anthozoa
[Orange fluorescent proteins from Anthozoa; dTomatoFP]
Orange fluorescent proteins from Anthozoa
[dummy term]
[E2GFP; photoswitchable fluorescent protein]
photoswitchable fluorescent protein
area detector
[EBFP; Blue fluorescent protein from Aequorea victoria]
Blue fluorescent protein from Aequorea victoria
[ECFP; Cyan fluorescent protein from Aequorea]
Cyan fluorescent protein from Aequorea
[electron microscopy; EDAX imaging]
electron microscopy
[electron microscopy; ESI electron spectroscopic imaging; EFTEM energy filtered TEM; EELS imaging]
electron microscopy
[EGFP; Green fluorescent protein from Aequorea]
Green fluorescent protein from Aequorea
[elastic scattering; imaged parameter]
imaged parameter
[elastic scattering of electrons]
elastic scattering
[elastic scattering of photons]
elastic scattering
[electron bombardment CCD (EBCCD); area detector]
[electron dense label; vizualization of label conjugated to probe]
vizualization of label conjugated to probe
[imaged parameter; electron density]
imaged parameter
[mode of electron microscopy; illumination by electrons; makes_use_of; electron microscopy; microscopy with lenses]
microscopy with lenses
[electron multiplying CCD (EMCCD); area detector]
[stain with broad specificity; electron-dense stain]
stain with broad specificity
[imaged parameter; elevation]
imaged parameter
[sample preparation method; embedded tissue]
sample preparation method
area detector
[EmeraldFP; Green fluorescent protein from Aequorea]
Green fluorescent protein from Aequorea
[emission-depletion illumination; specimen_modifying_illumination]
specimen_modifying_illumination
[LysoTracker; endogenous substrate, acidotropic-fluor conjugate]
[endogenous substrate, diaminobenzidine]
[endogenous substrate, dye conjugate]
[endogenous substrate, lectin-fluor conjugate]
[endogenous substrate, lectin-gold conjugate]
[endogenous substrate, non-vital dye]
[endogenous substrate, phalloidin-fluor conjugate]
[endogenous substrate, vital dye]
[enzyme label; vizualization of label conjugated to probe]
vizualization of label conjugated to probe
Staining with an aqueous or alcoholic solution of eosin Y,colors eosinophilic structures in various shades of red, pink and orange. The eosinophilic structures are generally composed of intracellular or extracellular protein. The Lewy bodies and Mallory bodies are examples of eosinophilic structures. Most of the cytoplasm is eosinophilic. Red blood cells are stained intensely red.
Staining with an aqueous or alcoholic solution of eosin Y,colors eosinophilic structures in various shades of red, pink and orange. The eosinophilic structures are generally composed of intracellular or extracellular protein. The Lewy bodies and Mallory bodies are examples of eosinophilic structures. Most of the cytoplasm is eosinophilic. Red blood cells are stained intensely red.
[eosin phalloidin fill]
[Red fluorescent proteins from Anthozoa; eqFP611]
Red fluorescent proteins from Anthozoa
[enzyme label; esterase]
enzyme label
[ethanol fixed tissue; coagulating-fixative fixed tissue]
coagulating-fixative fixed tissue
[membrane impermeant probe; ethidium bromide]
membrane impermeant probe
[EGS; ethylene glycol-bis-succinimidyl succinate fixed tissue; crosslinking-fixative fixed tissue]
crosslinking-fixative fixed tissue
[evanescent wave microscopy; total internal reflection fluorescence TIRF; fluorescence microscopy]
fluorescence microscopy
[FTIR frustrated total internal reflection; dark-field microscopy; evanescent wave scattering]
dark-field microscopy
[3D-deconvolution; exhaustive photon reassignment]
3D-deconvolution
[exogenous label]
[Yellow fluorescent protein from Aequorea; EYFP]
Yellow fluorescent protein from Aequorea
[fluorescent protein derived from Anthozoa; Far-red fluorescent proteins from Anthozoa]
fluorescent protein derived from Anthozoa
[farfield illumination; illumination method]
illumination method
[Fast-FT; fluorescent protein timer]
fluorescent protein timer
A method of reconstructing 3D structure by combining focussed ion beam milling to remove sucessive layers from a sample block with scanning EM to image the exposed surface between each milling step.
[area detector; film]
area detector
[FIONA (fluorescence imaging with one nanometer accuracy); point-localization method]
point-localization method
fluorescent label
[sample preparation method; fixation method]
sample preparation method
[FLAG peptide tag; small genetically encoded tag]
small genetically encoded tag
[FLIM; fluorescence microscopy]
fluorescence microscopy
Fluorescence loss in photobleaching; a method of determining whether two populations of fluorophore are in diffusive communication with one another by observing the decrease in brightness of one population when the other population is subjected to continuous photobleaching.
Fluorescence loss in photobleaching; a method of determining whether two populations of fluorophore are in diffusive communication with one another by observing the decrease in brightness of one population when the other population is subjected to continuous photobleaching.
[fluor labeled protein fill; fluor conjugated actin fill]
fluor labeled protein fill
[fluor labeled protein fill; fluor conjugated tubulin fill]
fluor labeled protein fill
[fluor labeled DNA probe]
[intracellular filling; fluor labeled protein fill]
intracellular filling
[intracellular filling; fluor labeled RNA fill]
intracellular filling
[fluorescent label; Fluorescein (FITC)]
[fluoresceine actin fill]
[fluoresceine phalloidin fill]
[fluoresceine-12-dUTP labeled DNA probe]
[imaged parameter; fluorescence emission]
imaged parameter
[fluorescence emission; UV/visible/IR illumination; makes_use_of; fluorescence microscopy; light microscopy]
light microscopy
[fluorescence polarization (polarization angle resolved fluorescence emission)]
fluorescence emission
[fluorescence microscopy; fluorescence polarization microscopy]
fluorescence microscopy
[intracellular filling; fluorescent dextran fill]
intracellular filling
[fluorescent label; vizualization of label conjugated to probe]
vizualization of label conjugated to probe
[fluorescent protein tag; fluorescent protein derived from Aequorea victoria]
fluorescent protein tag
[fluorescent protein derived from Anthozoa; fluorescent protein tag]
fluorescent protein tag
[fluorescent protein tag; fluorescent protein derived from Arabidopsis]
fluorescent protein tag
[fluorescent protein tag; genetically encoded tag]
genetically encoded tag
[optical highlighter; fluorescent protein timer]
optical highlighter
[fluoroscein labeled RNA probe]
A method of reconstructing 3D structure by combining focussed ion beam milling to remove sucessive layers from a sample block with scanning EM to image the exposed surface between each milling step.
[source of contrast; form birefringence]
source of contrast
[formaldehyde fixed tissue; crosslinking-fixative fixed tissue]
crosslinking-fixative fixed tissue
Fluorescence loss in photobleaching; a method of determining the rate and extent of exchange between a localized population of fluorophore and a non-localized diffusible pool, by observing the rate of recovery of brightness of the localized population after photobleaching.
Fluorescence loss in photobleaching; a method of determining the rate and extent of exchange between a localized population of fluorophore and a non-localized diffusible pool, by observing the rate of recovery of brightness of the localized population after photobleaching.
[free hand graphic; graphic illustration]
graphic illustration
[cryofixed tissue; freeze_fracture/freeze_etch]
cryofixed tissue
[cryofixed tissue; freeze-substituted tissue]
cryofixed tissue
Fluorescence (or Forster) resonance energy transfer; a method of estimating the distance between two fluorophores by measuring the extent of non-radiative energy transfer between the two. Experimentally, this involves selective excitation of one while observing emission from the other.
Fluorescence (or Forster) resonance energy transfer; a method of estimating the distance between two fluorophores by measuring the extent of non-radiative energy transfer between the two. Experimentally, this involves selective excitation of one while observing emission from the other.
[fuchsin; optically-dense stain]
optically-dense stain
[x-ray/gamma ray illumination; gamma ray illumination]
x-ray/gamma ray illumination
[genetically encoded biotin tag; small genetically encoded tag]
small genetically encoded tag
[genetically encoded enzyme; genetically encoded tag]
genetically encoded tag
[macromolecular probe; genetically encoded tag]
macromolecular probe
[giemsa; optically-dense stain]
optically-dense stain
[enzyme label; glucose oxidase]
enzyme label
[glutaraldehyde fixed tissue; crosslinking-fixative fixed tissue]
crosslinking-fixative fixed tissue
tissue made permeable by the action of glycerol
tissue made permeable by the action of glycerol
[gold; electron dense label]
electron dense label
[portrayed image; graphic illustration]
portrayed image
[graphite pencil graphic; line art graphic]
line art graphic
[fluorescent protein derived from Aequorea victoria; Green fluorescent protein from Aequorea]
fluorescent protein derived from Aequorea victoria
[fluorescent protein derived from Anthozoa; Green fluorescent proteins from Anthozoa]
fluorescent protein derived from Anthozoa
[grey scale graphic; computer graphic]
computer graphic
[resolution-enhancing method; ground state depletion scanning (GSD)]
resolution-enhancing method
[small genetically encoded tag; HA peptide tag]
small genetically encoded tag
[hand-sectioned tissue]
sectioned tissue
[Far-red fluorescent proteins from Anthozoa; HcRed-tandemFP]
Far-red fluorescent proteins from Anthozoa
[HcRed1FP; Red fluorescent proteins from Anthozoa]
Red fluorescent proteins from Anthozoa
[heat fixed tissue; fixation method]
fixation method
[lectin; Helix pomatia agglutinin]
The staining method involves application of hemalum, which is a complex formed from aluminium ions and oxidized haematoxylin. This colors nuclei of cells (and a few other objects, such as keratohyalin granules) blue. The staining of nuclei by hemalum does not require the presence of DNA and is probably due to binding of the dye-metal complex to arginine-rich basic nucleoproteins such as histones. The mechanism is different from that of nuclear staining by basic (cationic) dyes such as thionine or toluidine blue. Staining by basic dyes is prevented by chemical or enzymatic extraction of nucleic acids. Such extractions do not prevent staining of nuclei by hemalum.
The staining method involves application of hemalum, which is a complex formed from aluminium ions and oxidized haematoxylin. This colors nuclei of cells (and a few other objects, such as keratohyalin granules) blue. The staining of nuclei by hemalum does not require the presence of DNA and is probably due to binding of the dye-metal complex to arginine-rich basic nucleoproteins such as histones. The mechanism is different from that of nuclear staining by basic (cationic) dyes such as thionine or toluidine blue. Staining by basic dyes is prevented by chemical or enzymatic extraction of nucleic acids. Such extractions do not prevent staining of nuclei by hemalum.
[transmission electron microscopy (TEM); high-voltage electron microscopy (HVEM)]
[membrane-permeant probe; Hoechst 33258]
membrane-permeant probe
[Hoechst 33342; HO342; membrane-permeant probe]
membrane-permeant probe
[modified objective lens; Hoffman modulation]
modified objective lens
illumination parallel or at small angles to the optical axis is attenuated or blocked by a stop
illumination parallel or at small angles to the optical axis is attenuated or blocked by a stop
[enzyme label; horseradish peroxidase]
enzyme label
transmission electron microscopy (TEM)
area detector
[illumination by acoustic waves; illumination method]
illumination method
[illumination by electrons; illumination method]
illumination method
[illumination method; illumination by ions]
illumination method
[illumination method; illumination by neutrons]
illumination method
[illumination by photons; illumination method]
illumination method
sources and methods for illumination
sources and methods for illumination
The physical property of the specimen that leads to the modifications of the illumination that are recorded in the image.
The physical property of the specimen that leads to the modifications of the illumination that are recorded in the image.
[imaging method; method involved in biological imaging]
method involved in biological imaging
[modified objective lens; in-focus phase contrast]
modified objective lens
[incoherent illumination; illumination method]
illumination method
[imaged parameter; inelastic scattering]
imaged parameter
[inelastic scattering of electrons]
inelastic scattering
[inelastic scattering of photons (Raman scattering)]
[area detector; intensified CCD (ICCD)]
[intensified SIT (ISIT); area detector]
[optical method; interference contrast]
optical method
[optical method; interference reflection contrast (IRM)]
[intermediate voltage electron microacopy (IVEM); transmission electron microscopy (TEM)]
visualization of the connected regions of a cell by filling with a label
visualization of the connected regions of a cell by filling with a label
[source of contrast; intrinsic birefringence]
source of contrast
[source of contrast; intrinsic mass distribution]
source of contrast
[photoconvertible/photoswitchable fluorescent protein; IrisFP]
photoconvertible/photoswitchable fluorescent protein
optical method
area detector
A fragment of a cell, subcellular organelle, or macromolecular complex.
A fragment of a cell, subcellular organelle, or macromolecular complex.
transmission electron microscopy (TEM)
[Red fluorescent proteins from Anthozoa; JRedFP]
Red fluorescent proteins from Anthozoa
[photoconvertible fluorescent protein; KaedeFP]
photoconvertible fluorescent protein
[Far-red fluorescent proteins from Anthozoa; KatushkaFP]
Far-red fluorescent proteins from Anthozoa
[photoswitchable fluorescent protein; KFP1]
photoswitchable fluorescent protein
[Orange fluorescent proteins from Anthozoa; Kusabira OrangeFP]
Orange fluorescent proteins from Anthozoa
[Orange fluorescent proteins from Anthozoa; Kusabira OrangeFP2]
Orange fluorescent proteins from Anthozoa
[labeled nucleic acid probe]
[labeled primary antibody]
[electron-dense stain; lead salt]
electron-dense stain
[lectin; macromolecular probe]
macromolecular probe
[mode of light microscopy; UV/visible/IR illumination; makes_use_of; microscopy with lenses; light microscopy]
microscopy with lenses
illumination in the form of a thin sheet of light directed perpendicular to the optic axis
illumination in the form of a thin sheet of light directed perpendicular to the optic axis
[graphic illustration; line art graphic]
graphic illustration
a computational method that is linear in the mathematical sense, meaning that image intensity is conserved and the data remain suitable for all quantitative analyses
a computational method that is linear in the mathematical sense, meaning that image intensity is conserved and the data remain suitable for all quantitative analyses
Unfixed fully hydrated tissue, typically employed to monitor dynamic changes in the living state.
Unfixed fully hydrated tissue, typically employed to monitor dynamic changes in the living state.
[lucifer yellow; miscellaneous fill]
miscellaneous fill
specimen from which water has been removed by evaporation under vacuum at a temperature below freezing.
specimen from which water has been removed by evaporation under vacuum at a temperature below freezing.
[visualization by chemical attribute; macromolecular probe]
visualization by chemical attribute
methods for imaging objects large enough to be observed by the unaided eye
methods for imaging objects large enough to be observed by the unaided eye
[Red fluorescent proteins from Anthozoa; mAppleFP]
Red fluorescent proteins from Anthozoa
[3D-deconvolution; maximum likelihood deconvolution]
3D-deconvolution
[Red fluorescent proteins from Anthozoa; mCherryFP]
Red fluorescent proteins from Anthozoa
[mCyFP; Cyan fluorescent proteins from Anthozoa]
Cyan fluorescent proteins from Anthozoa
[Medium-FT; fluorescent protein timer]
fluorescent protein timer
[membrane impermeant probe; probes for nucleic acid]
probes for nucleic acid
[probes for nucleic acid; membrane-permeant probe]
probes for nucleic acid
[photoconvertible fluorescent protein; mEOS2FP]
photoconvertible fluorescent protein
[visualization by chemical attribute; metabolically incorporated radioisotope]
visualization by chemical attribute
[coagulating-fixative fixed tissue; methanol fixed tissue]
coagulating-fixative fixed tissue
[method involved in biological imaging]
[membrane impermeant probe; methyl green]
membrane impermeant probe
[methyl violet; optically-dense stain]
optically-dense stain
[methylene blue; optically-dense stain]
optically-dense stain
methods for forming images of objects too small to be observed with the unaided eye
methods for forming images of objects too small to be observed with the unaided eye
[microscopy with lenses]
microscopy
[microtome-sectioned tissue]
sectioned tissue
[phototropin 2; miniSOG]
phototropin 2
[miscellaneous fill; intracellular filling]
intracellular filling
[mKate2FP; Far-red fluorescent proteins from Anthozoa]
Far-red fluorescent proteins from Anthozoa
[mKateFP; Far-red fluorescent proteins from Anthozoa]
Far-red fluorescent proteins from Anthozoa
[photoconvertible fluorescent protein; mKikGR]
photoconvertible fluorescent protein
Uses an objective with extra components to manipulate the wave front in the plane of the objective aperture.
Uses an objective with extra components to manipulate the wave front in the plane of the objective aperture.
[negative staining; molybdenum compound]
negative staining
[portrayed image; montage]
portrayed image
[Orange fluorescent proteins from Anthozoa; mOrange2FP]
Orange fluorescent proteins from Anthozoa
[Orange fluorescent proteins from Anthozoa; mOrangeFP]
Orange fluorescent proteins from Anthozoa
[Far-red fluorescent proteins from Anthozoa; mPlumFP]
Far-red fluorescent proteins from Anthozoa
[Red fluorescent proteins from Anthozoa; mRasberryFP]
Red fluorescent proteins from Anthozoa
[Red fluorescent proteins from Anthozoa; mRFP1]
Red fluorescent proteins from Anthozoa
[Red fluorescent proteins from Anthozoa; mRubyFP]
Red fluorescent proteins from Anthozoa
[Red fluorescent proteins from Anthozoa; mStrawberryFP]
Red fluorescent proteins from Anthozoa
[mTagBFP; Blue fluorescent proteins from Anthozoa]
Blue fluorescent proteins from Anthozoa
[mTFP0.7; photoswitchable fluorescent protein]
photoswitchable fluorescent protein
[mTFP1; Cyan fluorescent proteins from Anthozoa]
Cyan fluorescent proteins from Anthozoa
[fluorescence emission; pulsed illumination; makes_use_of; narrowfield illumination; multi-photon microscopy; fluorescence microscopy]
fluorescence microscopy
illumination by multiple points of light simultaneously
illumination by multiple points of light simultaneously
[mWasabiFP; Green fluorescent proteins from Anthozoa]
Green fluorescent proteins from Anthozoa
[narrow field light micrograph]
[narrow-field detection; detection method]
detection method
illumination restricted to a small portion of the field of view of the imaging lens; often one or more diffraction limited spots
illumination restricted to a small portion of the field of view of the imaging lens; often one or more diffraction limited spots
[non-linear method; nearest-neighbors deblurring]
non-linear method
[nearfield illumination; illumination method]
illumination method
[makes_use_of; evanescent wave microscopy; nearfield illumination; illumination method]
illumination method
[makes_use_of; nearfield illumination; illumination method; evanescent wave scattering]
illumination method
[nearfield scanning optical microscopy (ANSOM); scanning probe microscopy]
visualization by exclusion of a stain in which the specimen is immersed; compare with “positive staining.
visualization by exclusion of a stain in which the specimen is immersed; compare with "positive staining.
[Nile blue A; optically-dense stain]
optically-dense stain
[Nile red; probe for lipid]
probe for lipid
[nitro blue tetrazolium chloride; optically-dense stain]
optically-dense stain
[non-linear method; no-neighbors deblurring]
non-linear method
[non-immunological protein probe; macromolecular probe]
macromolecular probe
computational method that are mathematically non-linear; image intensity is not conserved, and the data are in general not suitable for quantitative analyses of pixel values
computational method that are mathematically non-linear; image intensity is not conserved, and the data are in general not suitable for quantitative analyses of pixel values
[probe for protein; non-specific protein affinity]
probe for protein
[optically-dense stain; nuclear fast red]
optically-dense stain
[oblique illumination; illumination method]
illumination method
optical coherence tomography; an interferometric method of imaging using back-scattered photons (elastic scattering)
optical coherence tomography; an interferometric method of imaging using back-scattered photons (elastic scattering)
[oil painted graphic]
painted graphic
[optical highlighter; fluorescent protein tag]
fluorescent protein tag
[optical method; contrast-enhancing method]
contrast-enhancing method
[imaged parameter; optical path length gradient]
imaged parameter
[stain with broad specificity; optically-dense stain]
stain with broad specificity
[fluorescent protein derived from Anthozoa; Orange fluorescent proteins from Anthozoa]
fluorescent protein derived from Anthozoa
[non-specific protein affinity; orcein]
non-specific protein affinity
[Oregon Green 488 phalloidin fill]
[Oregon Green 488-5-dUTP labeled DNA probe]
[Oregon Green 514 phalloidin fill]
[Oregon Green 514 tubulin fill]
[small-molecule probe; organelle-specific probe]
small-molecule probe
[orientation-independent polarization microscopy]
polarization microscopy
[osmium tetroxide; electron-dense stain]
electron-dense stain
[osmium tetroxide fixed tissue; crosslinking-fixative fixed tissue]
crosslinking-fixative fixed tissue
[other negative stain; negative staining]
negative staining
[other radioisotope; metabolically incorporated radioisotope]
metabolically incorporated radioisotope
[PA-CFP; photoactivatable fluorescent protein]
photoactivatable fluorescent protein
[PA-GFP; photoactivatable fluorescent protein]
photoactivatable fluorescent protein
[PA-mCherry1FP; photoactivatable fluorescent protein]
photoactivatable fluorescent protein
[PA-mRFP; photoactivatable fluorescent protein]
photoactivatable fluorescent protein
[PadronFP; photoswitchable fluorescent protein]
photoswitchable fluorescent protein
[PAINT (point accumulation for imaging in nanoscale topography); point-localization method]
point-localization method
[painted graphic; line art graphic]
line art graphic
[makes_use_of; PALM (photoactivation localization microscopy); point-localization method; photo-activation illumination]
point-localization method
[pastel painted graphic]
painted graphic
[black and white graphic; pen and ink graphic; line art graphic]
line art graphic
[peptide-nucleic acid probe; sequence-specific nucleic acid probe]
sequence-specific nucleic acid probe
Tissue treated to make cell membranes permeable, typically for the purpose of allowing access by exogenous stains or substrates.
Tissue treated to make cell membranes permeable, typically for the purpose of allowing access by exogenous stains or substrates.
[affinity for specific proteins; phalloidin]
affinity for specific proteins
[Phamret; photoactivatable fluorescent protein]
photoactivatable fluorescent protein
[phase contrast microscopy; makes_use_of; refractive index; light microscopy; hollow-cone illumination]
light microscopy
[Phaseolus vulgaris lectin PHA-L)]
[Yellow fluorescent proteins from Anthozoa; PhiYFP]
Yellow fluorescent proteins from Anthozoa
[specimen_modifying_illumination; photo-ablation illumination]
specimen_modifying_illumination
[specimen_modifying_illumination; photo-activation illumination]
specimen_modifying_illumination
[specimen_modifying_illumination; photo-bleaching illumination]
specimen_modifying_illumination
[specimen_modifying_illumination; photo-switching illumination]
specimen_modifying_illumination
[optical highlighter; photoactivatable fluorescent protein]
optical highlighter
[photoconvertible fluorescent protein; optical highlighter]
optical highlighter
[optical highlighter; photoconvertible/photoswitchable fluorescent protein]
optical highlighter
[photodiode; spot detector]
spot detector
[photomultiplier tube (PMT); spot detector]
[optical highlighter; photoswitchable fluorescent protein]
optical highlighter
[fluorescent protein derived from Arabidopsis; phototropin 2]
fluorescent protein derived from Arabidopsis
[piezo-electric device; spot detector]
spot detector
spot detector
lectin
[resolution-enhancing method; point-localization method]
resolution-enhancing method
[optical method; polarization contrast]
optical method
[retardance; polarized light illumination; polarization-sensitive detection; makes_use_of; polarization microscopy; light microscopy]
light microscopy
[detection method; polarization-sensitive detection]
detection method
[illumination by photons; polarized light illumination]
illumination by photons
methods and devices used for creating images by some form of artwork, as distinct from capturing images by means of some recording device
methods and devices used for creating images by some form of artwork, as distinct from capturing images by means of some recording device
[potassium permanganate; electron-dense stain]
electron-dense stain
[primary antibody plus labeled secondary antibody]
[primary antibody-alkaline phosphastase conjugate, DAB]
[primary antibody-alkaline phosphastase conjugate, ELF 97 phosphate]
[primary antibody-alkaline phosphastase conjugate, Vector Black]
[primary antibody-alkaline phosphastase conjugate, Vector Blue]
[primary antibody-alkaline phosphastase conjugate, Vector Red]
[AMCA; primary antibody-aminomethylcoumarin acetate]
[primary antibody-Cascade blue]
[Cy2; primary antibody-cyanine]
[primary antibody-enzyme conjugate, substrate]
[primary antibody-fluor conjugate]
[FITC; primary antibody-fluorescein isothiocyanate]
[primary antibody-gold conjugate]
[primary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole]
[primary antibody-horseradish peroxidase, 3,3’,5,5’-tetramethylbenzidine]
[primary antibody-horseradish peroxidase, 4-chloro-1-naphthol]
[primary antibody-horseradish peroxidase, Vector NovaRed]
[primary antibody-horseradish peroxidase, Vector SG]
[primary antibody-horseradish peroxidase, Vector VIP]
[primary antibody-indocarbocyanine; Cy3]
[primary antibody-indodicarbocyanine; Cy5]
[RRX; primary antibody-rhodamine red-X]
[TRITC; primary antibody-tetramethyl rhodamine isothiocyanate]
[primary antibody-Texas Red; TR]
[probe for endoplasmic reticulum; organelle-specific probe]
organelle-specific probe
[probe for Golgi; organelle-specific probe]
organelle-specific probe
[small-molecule probe; probe for lipid]
small-molecule probe
[probe for lysosomes; organelle-specific probe]
organelle-specific probe
[probe for mitochondria; organelle-specific probe]
organelle-specific probe
[probe for protein; small-molecule probe]
small-molecule probe
[small-molecule probe; probes for nucleic acid]
small-molecule probe
[membrane impermeant probe; PI; propidium iodide]
membrane impermeant probe
illumination that varies with time; often achieved with pulsed lasers for the purpose of generating very-high peak illumination power
illumination that varies with time; often achieved with pulsed lasers for the purpose of generating very-high peak illumination power
[pyronine; membrane-permeant probe]
membrane-permeant probe
An imaging technique that uses high energy electromagnetic radiation, typically X-rays, as a source of illumination.
An imaging technique that uses high energy electromagnetic radiation, typically X-rays, as a source of illumination.
[radioisotope labeled DNA probe]
[radioisotope labeled RNA probe]
inelastic scattering
methods and devices used for capturing an image of a real object; distinct from images created by artwork
methods and devices used for capturing an image of a real object; distinct from images created by artwork
[Red fluorescent proteins from Anthozoa; fluorescent protein derived from Anthozoa]
fluorescent protein derived from Anthozoa
[imaged parameter; refractive index]
imaged parameter
methods for increasing the resolution beyond the classical Abbe diffraction limit
methods for increasing the resolution beyond the classical Abbe diffraction limit
[imaged parameter; retardance]
imaged parameter
illumination at angles less than the acceptance angle of the image-forming lens is attenuated by a filter to give a colored background of unscattered light; higher angle illumination may be filtered to give a contrasting color
illumination at angles less than the acceptance angle of the image-forming lens is attenuated by a filter to give a colored background of unscattered light; higher angle illumination may be filtered to give a contrasting color
[Rhodamine; fluorescent label]
fluorescent label
[rhodamine 123; probe for mitochondria]
probe for mitochondria
[rhodamine actin fill]
[rhodamine B; optically-dense stain]
optically-dense stain
[rhodamine phalloidin fill]
[sequence-specific nucleic acid probe; RNA probe]
sequence-specific nucleic acid probe
[rsCherryFP; photoswitchable fluorescent protein]
photoswitchable fluorescent protein
[photoswitchable fluorescent protein; rsCherryRevFP]
photoswitchable fluorescent protein
[rsFastLimeFP; photoswitchable fluorescent protein]
photoswitchable fluorescent protein
[sample preparation method; method involved in biological imaging]
method involved in biological imaging
Saponin, a detergent-like molecule, preferentially forms holes in membranes that contain cholesterol.
Saponin, a detergent-like molecule, preferentially forms holes in membranes that contain cholesterol.
structured illumination used at sufficiently high power to cause significant ground-state depletion of the imaged fluorophore, thus allowing for non-linear resolution enhancement
A method of reconstructing 3D structure by combining serial sectioning with scanning EM.
image is formed by scanning the surface of the specimen with a beam of electrons in a raster pattern
imaging by means of a physical probe that passes over the sample
imaging by means of a physical probe that passes over the sample
[scanning tunneling microscopy; scanning probe microscopy]
scanning probe microscopy
[scanning-transmission electron microscopy]
electron microscopy
[Cyan fluorescent protein from Aequorea; SCFP]
Cyan fluorescent protein from Aequorea
[secondary antibody-Alexa Fluor conjugate]
[secondary antibody-alkaline phosphastase conjugate, BCIP]
[secondary antibody-alkaline phosphastase conjugate, DAB]
[secondary antibody-alkaline phosphastase conjugate, ELF 97 phosphate]
[secondary antibody-alkaline phosphastase conjugate, Fast Blue B]
[secondary antibody-alkaline phosphastase conjugate, Fast Red]
[secondary antibody-alkaline phosphastase conjugate, INT]
[secondary antibody-alkaline phosphastase conjugate, Magenta Phos]
[secondary antibody-alkaline phosphastase conjugate, NABP]
[secondary antibody-alkaline phosphastase conjugate, NAGP]
[secondary antibody-alkaline phosphastase conjugate, NAMP]
[secondary antibody-alkaline phosphastase conjugate, NATP]
[secondary antibody-alkaline phosphastase conjugate, NBT]
[secondary antibody-alkaline phosphastase conjugate, Vector Black]
[secondary antibody-alkaline phosphastase conjugate, Vector Blue]
[secondary antibody-alkaline phosphastase conjugate, Vector Red]
[secondary antibody-beta-galactosidase, X-Gal]
[secondary antibody-beta-glucuronidase, X-gluc]
[Cy2; secondary antibody-cyanine conjugate]
[secondary antibody-diaminotriazinylaminoflorescein conjugate; DTAF]
[secondary antibody-enzyme conjugate, substrate]
[secondary antibody-esterase, Naphthol-AS-D-chloroacetate]
[secondary antibody-fluor conjugate]
[secondary antibody-fluorescein isothiocyanate conjugate; FITC]
[secondary antibody-glucose oxidase, TNBT]
[secondary antibody-horseradish peroxidase, 3-amino-9-ethylcarbazole]
[secondary antibody-horseradish peroxidase, 3,3’-diaminobenzidine]
[secondary antibody-horseradish peroxidase, 3,3’,5,5’-tetramethylbenzidine]
[secondary antibody-horseradish peroxidase, 3,3'-diaminobenzidine silver enhanced]
[secondary antibody-horseradish peroxidase, 4-chloro-1-naphthol]
[secondary antibody-horseradish peroxidase, Vector NovaRed]
[secondary antibody-horseradish peroxidase, Vector SG]
[secondary antibody-horseradish peroxidase, Vector VIP]
[secondary antibody-indocarbocyanine conjugate; Cy3]
[Cy5; secondary antibody-indodicarbocyanine conjugate]
[LSRC; secondary antibody-lissamine rhodamine sulfonyl chloride]
[secondary antibody-phosphatase, Naphthol-AS-BI-phosphate]
[secondary antibody-rhodamine red-X conjugate; RRX]
[secondary antibody-tetramethyl rhodamine isothiocyanate conjugate; TRITC]
[secondary antibody-Texas red conjugate; TR]
electrons generated by inelastic scattering of other radiation, the primary radiation.
electrons generated by inelastic scattering of other radiation, the primary radiation.
image created using the electrons generated by ionization of a sample due to inelastic scattering of the primary beam of radiation
image created using the electrons generated by ionization of a sample due to inelastic scattering of the primary beam of radiation
[sample preparation method; sectioned tissue]
sample preparation method
image is formed by scanning the surface of the specimen with a beam of electrons in a raster pattern
[sequence-specific nucleic acid probe; macromolecular probe]
macromolecular probe
A method of reconstructing 3D structure by combining serial sectioning with scanning EM.
visualization by depositing a label on the surface of a specimen uniformly (plating), or from a particular direction so that surface topography becomes visible (shadowing)
visualization by depositing a label on the surface of a specimen uniformly (plating), or from a particular direction so that surface topography becomes visible (shadowing)
[area detector; silicon intensified target tube (SIT)]
[silver-intensified cobalt fill]
cobalt fill
resolution-enhancing method
illumination by a single spot of light that is scanned across the field of view
illumination by a single spot of light that is scanned across the field of view
[single sideband edge enhancement (SSBE); modified objective lens]
[makes_use_of; single-spot confocal microscopy; single point scanning; spot detector]
confocal microscopy
[Blue fluorescent protein from Aequorea victoria; SiriusFP]
Blue fluorescent protein from Aequorea victoria
area detector
illumination and detection via a thin slit
illumination and detection via a thin slit
illumination in the shape of a thin line that is swept over the field of view
illumination in the shape of a thin line that is swept over the field of view
[fluorescent protein timer; Slow-FT]
fluorescent protein timer
[small genetically encoded tag]
genetically encoded tag
[small-molecule probe; visualization by chemical attribute]
visualization by chemical attribute
permeabilization due to extraction of lipid from membranes by an organic solvent such as acetone or methanol.
permeabilization due to extraction of lipid from membranes by an organic solvent such as acetone or methanol.
The source of the variation in the imaged parameter across the field of view.
The source of the variation in the imaged parameter across the field of view.
[soybean agglutinin; lectin]
Illumination used for the purpose of inducing some observable change in the specimen during the course of an imaging session. This illumination may or may not be the same as the illumination used for imaging. Typically used in conjunction with live-cell imaging
Illumination used for the purpose of inducing some observable change in the specimen during the course of an imaging session. This illumination may or may not be the same as the illumination used for imaging. Typically used in conjunction with live-cell imaging
single (or selective) plane illumination.
single (or selective) plane illumination.
[spinning disk confocal microscopy; charge coupled device (CCD); narrow-field detection; makes_use_of; spinning disk scanning]
confocal microscopy
scanning by multiple points of light arising from pinholes in a spinning disk that is illuminated by a widefield source
scanning by multiple points of light arising from pinholes in a spinning disk that is illuminated by a widefield source
[spot detector; detection method]
detection method
modified objective lens
structured illumination used at sufficiently high power to cause significant ground-state depletion of the imaged fluorophore, thus allowing for non-linear resolution enhancement
[small-molecule probe; stain with broad specificity; positive staining]
small-molecule probe
resolution-enhancing method
[stiffness; imaged parameter]
imaged parameter
[emission-depletion illumination; resolution-enhancing method; makes_use_of; stimulated emission depletion (STED); narrowfield illumination]
[makes_use_of; photo-switching illumination; point-localization method; STORM (stochastic optical reconstruction microscopy)]
point-localization method
[source of contrast; stress-induced birefringence]
source of contrast
[resolution-enhancing method; structured illumination microscopy (SIM)]
[sub-nanosecond time-resolved fluorescence emission)]
fluorescence emission
[SuperfolderFP; Green fluorescent protein from Aequorea]
Green fluorescent protein from Aequorea
[Yellow fluorescent protein from Aequorea; SYFP]
Yellow fluorescent protein from Aequorea
[SYTO Blue; membrane-permeant probe]
membrane-permeant probe
[SYTO Green; membrane-permeant probe]
membrane-permeant probe
[SYTO Orange; membrane-permeant probe]
membrane-permeant probe
[SYTO Red; membrane-permeant probe]
membrane-permeant probe
[SYTOX Green; membrane impermeant probe]
membrane impermeant probe
[T-SapphireFP; Green fluorescent protein from Aequorea]
Green fluorescent protein from Aequorea
[TagCFP; Cyan fluorescent proteins from Anthozoa]
Cyan fluorescent proteins from Anthozoa
[Green fluorescent proteins from Anthozoa; TagGFP]
Green fluorescent proteins from Anthozoa
[Green fluorescent proteins from Anthozoa; TagGFP2]
Green fluorescent proteins from Anthozoa
[Orange fluorescent proteins from Anthozoa; TagRFP]
Orange fluorescent proteins from Anthozoa
[TagRFP-T; Orange fluorescent proteins from Anthozoa]
Orange fluorescent proteins from Anthozoa
[Yellow fluorescent proteins from Anthozoa; TagYFP]
Yellow fluorescent proteins from Anthozoa
[taxol; affinity for specific proteins]
affinity for specific proteins
[tdKatuskaFP; Far-red fluorescent proteins from Anthozoa]
Far-red fluorescent proteins from Anthozoa
[Red fluorescent proteins from Anthozoa; tdRFP611]
Red fluorescent proteins from Anthozoa
[Far-red fluorescent proteins from Anthozoa; tdRFP639]
Far-red fluorescent proteins from Anthozoa
[tdTomatoFP; Orange fluorescent proteins from Anthozoa]
Orange fluorescent proteins from Anthozoa
electron microscopy
[tetracysteine tag; small genetically encoded tag]
small genetically encoded tag
fluorophore derivative of rhodamine with ex/em 547/572
[tetrodotoxin; affinity for specific proteins]
affinity for specific proteins
[Texas Red-X phalloidin fill]
fluorophore with ex/em 589/615
fluorophore with ex/em 589/615
[light microscopy; time lapse microscopy]
light microscopy
[tissue in acrylic resin embedment; tissue in plastic embedment]
tissue in plastic embedment
[tissue in plastic embedment; tissue in epoxy resin embedment]
tissue in plastic embedment
[tissue in wax embedment; tissue in paraffin embedment]
tissue in wax embedment
[tissue in plastic embedment; embedded tissue]
embedded tissue
[tissue in polyester wax embedment; tissue in wax embedment]
tissue in wax embedment
[tissue in polyethylene glycol embedment; tissue in wax embedment]
tissue in wax embedment
Fully hydrated tissue rapidly frozen to produce vitrified water, and maintained continuously below the glass transition temperature of water (approximately -137 deg C
Fully hydrated tissue rapidly frozen to produce vitrified water, and maintained continuously below the glass transition temperature of water (approximately -137 deg C
[tissue in wax embedment; embedded tissue]
embedded tissue
[toluidine blue; optically-dense stain]
optically-dense stain
An imaging technique that creates virtual sections through the use of any kind of penetrating wave.
An imaging technique that creates virtual sections through the use of any kind of penetrating wave.
[Yellow fluorescent protein from Aequorea; TopazFP]
Yellow fluorescent protein from Aequorea
[makes_use_of; transmission electron microscopy (TEM); wide-field detection; widefield illumination]
[specimen_modifying_illumination; trapping illumination]
specimen_modifying_illumination
fluorophore derivative of rhodamine with ex/em 547/572
[negative staining; tungsten compound]
negative staining
[Orange fluorescent proteins from Anthozoa; turboRFP]
Orange fluorescent proteins from Anthozoa
[Yellow fluorescent proteins from Anthozoa; TurboYFP]
Yellow fluorescent proteins from Anthozoa
[two-photon laser scanning microscopy; multi-photon microscopy]
multi-photon microscopy
[specimen_modifying_illumination; uncaging illumination]
specimen_modifying_illumination
[sample preparation method; unembedded tissue]
sample preparation method
[unfixed tissue; fixation method]
fixation method
data as acquired, with no alterations that change the pixel values.
data as acquired, with no alterations that change the pixel values.
[negative staining; uranium compound]
negative staining
[electron-dense stain; uranyl salt]
electron-dense stain
[illumination by photons; UV/visible/IR illumination]
illumination by photons
[Yellow fluorescent protein from Aequorea; VenusFP]
Yellow fluorescent protein from Aequorea
[vibratome-sectioned tissue]
sectioned tissue
[vidicon tube camera; area detector]
area detector
methods of visualization that differentiate regions of the specimen on the basis of their chemical properties
methods of visualization that differentiate regions of the specimen on the basis of their chemical properties
[visualization method; method involved in biological imaging]
method involved in biological imaging
methods of visualization that differentiate between different regions or surfaces of the specimen based on contiguity
methods of visualization that differentiate between different regions or surfaces of the specimen based on contiguity
[visualization method; vizualization of label conjugated to probe]
visualization method
[watercolor painted graphic]
painted graphic
[lectin; wheat germ agglutinin]
[sample preparation method; whole mounted tissue]
sample preparation method
[wide field light micrograph]
[wide-field detection; detection method]
detection method
illumination that covers the entire field of view of the image-forming lens
illumination that covers the entire field of view of the image-forming lens
[Wiener filter; linear method]
linear method
a birefringent optical element typically used to split polarized illumination into two sets of parallel but displaced rays
a birefringent optical element typically used to split polarized illumination into two sets of parallel but displaced rays
[photoconvertible fluorescent protein; wtEOSFP]
photoconvertible fluorescent protein
[photoconvertible fluorescent protein; wtKIikGR]
photoconvertible fluorescent protein
[X-ray illumination; X-ray CT; X-ray computed tomography; makes_use_of; CAT scan; CT scan; X-ray tomography; computed axial tomography]
X-ray tomography; computed tomography
[X-ray computed tomography]
[fluorescence emission; X-ray fluorescence]
fluorescence emission
[X-ray illumination; x-ray/gamma ray illumination]
x-ray/gamma ray illumination
[X-ray radiography; X-ray microscopy; X-ray illumination; makes_use_of]
X-ray radiography; microscopy
Radiography using X-rays
Radiography using X-rays
Tomography using X-ray illumination.
Tomography using X-ray illumination.
[x-ray/gamma ray illumination; illumination by photons]
illumination by photons
fluorophore derivative of rhodamine with ex/em 580/605
fluorophore derivative of rhodamine with ex/em 580/605
[fluor labeled RNA fill; X-rhodamine RNA fill]
fluor labeled RNA fill
[X-rhodamine tubulin fill]
[fluorescent protein derived from Aequorea victoria; Yellow fluorescent protein from Aequorea]
fluorescent protein derived from Aequorea victoria
[fluorescent protein derived from Anthozoa; Yellow fluorescent proteins from Anthozoa]
fluorescent protein derived from Anthozoa
[membrane impermeant probe; YO-PRO-1]
membrane impermeant probe
[yPet; Yellow fluorescent protein from Aequorea]
Yellow fluorescent protein from Aequorea
[Green fluorescent proteins from Anthozoa; ZsGreenFP]
Green fluorescent proteins from Anthozoa
[Yellow fluorescent proteins from Anthozoa; ZsYellowFP]
Yellow fluorescent proteins from Anthozoa
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